HEK293T Human CD268/BAFFR Stable Cell Line (HV599818)

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Overview

Catalog No.

HV599818

Brand

abinScience

Description

The HEK293T/Human BAFFR Stable Cell Line was engineered to express the full length human BAFFR (Uniprot ID: Q96RJ3). Surface expression of human BAFFR was confirmed by flow cytometry.

Accession

Q96RJ3

Species

Human

Host species

HEK293T

Target

CD268/BAFFR

Properties

Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)

Product Usage Information

Application	Dilution
Culture Medium	DMEM, 10% FBS, 1% P/S
Freeze Medium	FBS:DMSO=9:1

Stability and Storage

Frozen in liquid nitrogen.

Specifications

1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium

Background

Tumor necrosis factor receptor superfamily member 13C (CD268/BAFFR) is a ~18 kDa protein. B-cell receptor specific for TNFSF13B/TALL1/BAFF/BLyS. Promotes the survival of mature B-cells and the B-cell response.

HEK293T

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Flow cytometry

Flow cytometry validation of a BAFFR stable cell line using Ianalumab-APC. Human BAFFR-stable cells were incubated with APC-labeled Ianalumab (5 µg/ml) for 30 minutes at room temperature. After washing, cells were analyzed on a NovoCyte Flow Cytometer. APC-positive signal (yellow histogram) indicates specific binding of Ianalumab to BAFFR, whereas unstained cells (blue histogram) serve as a negative control, confirming surface expression of human BAFFR.

References

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