HEK293T Human CD66c/CEACAM6 Stable Cell Line (HW494818)

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Overview

Catalog No.	HW494818
Description	HEK293T Human CD66c/CEACAM6 Stable Cell Line (HW494818) is a stable cell line expressing CD66c/CEACAM6. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P40199
Applications	FCM
Species	Human
Host species	HEK293T
Target	CD66c/CEACAM6
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Cell adhesion molecule CEACAM6 (CD66c) is a ~37 kDa protein. Cell surface glycoprotein that plays a role in cell adhesion and tumor progression. Intercellular adhesion occurs in a calcium- and fibronectin-independent manner. Mediates homophilic and heterophilic cell adhesion with other carcinoembryonic antigen-related cell adhesion molecules, such as CEACAM5 and CEACAM8. Heterophilic interaction with CEACAM8 occurs in activated neutrophils. Plays a role in neutrophil adhesion to cytokine-activated endothelial cells. 1. Ordoñez, C. et al. (2000) Cancer research 60, 3419-24. PMID: 10910050 2. Kuroki, M. et al. (2001) Journal of leukocyte biology 70, 543-50. PMID: 11590190 3. Kuijpers, TW. et al. (1992) The Journal of cell biology 118, 457-66. PMID: 1378450 4. Blumenthal, RD. et al. (2005) Cancer research 65, 8809-17. PMID: 16204051 5. Oikawa, S. et al. (1991) The Journal of biological chemistry 266, 7995-8001. PMID: 2022629 6. Oikawa, S. et al. (1989) Biochemical and biophysical research communications 164, 39-45. PMID: 2803308 7. Yamanaka, T. et al. (1996) Protein expression and purification 7, 438-46. PMID: 8776764

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human CEACAM6 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

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