HEK293T Human VIPR1/VPAC1 Stable Cell Line (HB111818)

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Overview

Catalog No.	HB111818
Description	HEK293T Human VIPR1/VPAC1 Stable Cell Line (HB111818) is a stable cell line expressing VIPR1/VPAC1. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	P32241
Applications	FCM
Species	Human
Host species	HEK293T
Target	VIPR1/VPAC1
Detection method	Colorimetric
Sample type	Plasma, Serum
Assay type	Quantitative
Range	1.56 - 100 ng/mL
Sensitivity	0.25 ng/mL
Recovery	80-120%
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Shipping	2-8 ℃
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	Vasoactive intestinal polypeptide receptor 1 (VIPR1/VPAC1) is a ~51 kDa protein. G protein-coupled receptor activated by the neuropeptides vasoactive intestinal peptide (VIP) and pituitary adenylate cyclase-activating polypeptide (ADCYAP1/PACAP). Binds VIP and both PACAP27 and PACAP38 bioactive peptides with the following order of ligand affinity VIP = PACAP27 > PACAP38. Ligand binding causes a conformation change that triggers signaling via guanine nucleotide-binding proteins (G proteins) and modulates the activity of downstream effectors. Activates cAMP-dependent pathway. 1. Xu, Y. et al. (2022) Nature communications 13, 2272. PMID: 35477937 2. Piper, SJ. et al. (2022) Nature communications 13, 7013. PMID: 36385145 3. Couvineau, A. et al. (1994) Biochemical and biophysical research communications 200, 769-76. PMID: 8179610
Note	For research use only. This stable cell line expresses GFP as a reporter marker. GFP is expressed independently of the target protein and is not fused to the target protein.

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Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human VIPR1 monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet

HEK293T Human VIPR1/VPAC1 Stable Cell Line.pdf

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HEK293T Human VIPR1/VPAC1 Stable Cell Line (HB111818)

Overview

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References

Datasheet

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