HEK293T Human VSIR/VISTA Stable Cell Line (HP543818)

Overview

Catalog No.	HP543818
Description	HEK293T Human VSIR/VISTA Stable Cell Line (HP543818) is a stable cell line expressing VSIR/VISTA. Engineered for consistent, high-level target expression in research applications. Highlights ●Stable Expression — Engineered for consistent, high-level target expression.
Accession	Q9H7M9
Applications	FCM
Species	Human
Host species	HEK293T
Target	VSIR/VISTA
Properties	Growth Properties: Adherent; Selection Marker: Puromycin (1 μg/mL)
Stability and Storage	Frozen in liquid nitrogen.
Specifications	1 vial contains at least 2×10^6 cells in 1 mL Freeze Medium
Background	V-type immunoglobulin domain-containing suppressor of T-cell activation (VSIR/VISTA) is a ~33 kDa protein. Immunoregulatory receptor which inhibits the T-cell response. May promote differentiation of embryonic stem cells, by inhibiting BMP4 signaling. May stimulate MMP14-mediated MMP2 activation. 1. Lines, JL. et al. (2014) Cancer research 74, 1924-32. PMID: 24691993 2. Sakr, MA. et al. (2010) Cancer science 101, 2368-74. PMID: 20666777

Images

Flow cytometry

Untransfected cells (blue Histogram) and Transfected cells (Yellow Histogram) were stained with an anti-human VISTA monoclonal antibody at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.

References

Datasheet