InVivoMAb Anti-Human IL-6 Antibody (Iv0022) (HY328010)

Name: InVivoMAb Anti-Human IL-6 Antibody (Iv0022)
Brand: abinScience
SKU: HY328010
Price: 370 USD
Availability: InStock

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Spec:

100ug
1mg

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

HY328010

Description

InVivoMAb Anti-Human IL6 (Iv0022) [Iv0022] (HY328010) is a human monoclonal antibody detecting Hybridoma growth factor in ELISA, FCM, Neutralization. Suitable for Human.

Highlights

●Multi-Application — Validated across multiple applications.
●Flow Cytometry — Validated for flow cytometry applications.

Species reactivity

Human

Applications

ELISA, FCM, WB, Neutralization

Host species

Human

Isotype

IgG1, kappa

Clone ID

Iv0022

Clonality

Monoclonal

Tested applications

WB, FCM

Target

B-cell stimulatory factor 2, BSF-2, CDF, CTL differentiation factor, Hybridoma growth factor, IFN-beta-2, IFNB2, IL-6, IL6, Interferon beta-2, Interleukin-6

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Associated products

pH 7.4 Dilution Buffer

Purification

Protein A/G purified from cell culture supernatant.

Accession

P05231

Form

Liquid

Storage buffer

0.01M PBS pH 7.4

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Reconstitution

Reconstitute in sterile water for a stock solution. A copy of datasheet will be provided with the products, please refer to it for details.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Note

For research use only. Not suitable for clinical or therapeutic use.

Isotype Control

Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

Images

Bioactivity

SEC-HPLC detection for InVivoMAb Anti-Human IL6 (ABV0244).
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.IL6 Transfected CHO cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
Flow Cytometry

Flow-cytometry using anti-human IL6 antibody.LPS treated THP-1 cells were stained with an irrelevant antibody (Blue Histogram) or an anti-human IL6 antibody monoclonal antibody (Catalog # HY328010 ,Green Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, bound antibody was detected using a FITC conjugated goat anti-human antibody (Catalog # HF690414) and cells analysed on a NovoCyte Flow Cytometer.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Human IL6 (Iv0022).
Western blot

Western blot analysis was performed using anti-IL6 monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human IL6 (Catalog No: HY328021), 50ng
Lane 2: recombinant human IL6 (Catalog No: HY328021), 30ng
Lane 3: recombinant human IL6 (Catalog No: HY328021), 20ng
Lane 4: recombinant human IL6 (Catalog No: HY328021), 10ng