Human CD239/BCAM Recombinant Protein (C-His) (HW406031)

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Catalog No.	HW406031
Description	Recombinant Human CD239/BCAM Protein, C-His (HW406031) expressed in Mammalian Cells, spanning Met1-Gly548. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P50895
Protein length	Met1-Gly548
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>95% as determined by SDS-PAGE.
Predicted molecular weight	60.35 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Basal cell adhesion molecule, LU, MSK19, Auberger B antigen, CD239, Lutheran blood group glycoprotein, Lutheran antigen, F8/G253 antigen, B-CAM cell surface glycoprotein, BCAM
Background	Basal cell adhesion molecule (CD239/BCAM) is a ~67 kDa protein. Transmembrane glycoprotein that functions as both a receptor and an adhesion molecule playing a crucial role in cell adhesion, motility, migration and invasion. Extracellular domain enables binding to extracellular matrix proteins, such as laminin, integrin and other ligands while its intracellular domain interacts with cytoskeletal proteins like hemoglobin, facilitating cell signal transduction. Serves as a receptor for laminin alpha-5/LAMA5 to promote cell adhesion. Mechanistically, JAK2 induces BCAM phosphorylation and activates its adhesion to laminin by stimulating a Rap1/AKT signaling pathway in the absence of EPOR. 1. Udani, M. et al. (1998) The Journal of clinical investigation 101, 2550-8. PMID: 9616226 2. Guadall, A. et al. (2019) The Journal of biological chemistry 294, 14911-14921. PMID: 31413112 3. El Nemer, W. et al. (2007) Blood 109, 3544-51. PMID: 17158232 4. Gauthier, E. et al. (2005) The Journal of biological chemistry 280, 30055-62. PMID: 15975931 5. De Grandis, M. et al. (2013) Blood 121, 658-65. PMID: 23160466
Note	For research use only.

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SDS-PAGE for Recombinant Human CD239/BCAM Protein, C-His

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Datasheet

Human CD239/BCAM Recombinant Protein (C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

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V₂ (Working Vol.)

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Human CD239/BCAM Recombinant Protein (C-His) (HW406031)

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