Human CD300f/CD300LF Recombinant Protein (C-His) (HV211011)

Name: Human CD300f/CD300LF Recombinant Protein (C-His)
Brand: abinScience
SKU: HV211011
Price: 298 USD
Availability: InStock

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Overview

Catalog No.	HV211011
Description	_x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Recombinant Human CD300f/CD300LF Protein, C-His (HV211011) expressed in Mammalian Cells, spanning Thr20-Leu155. Purity: >90% by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ Highlights _x005F_x000D_ _x005F_x000D_ _x005F_x000D_ ●His-Tagged — N-terminal 6×His tag for IMAC purification. _x005F_x000D_ ●Mammalian Expression — Proper folding and native glycosylation. _x005F_x000D_ ●High Purity — >90% purity verified by SDS-PAGE. _x005F_x000D_ _x005F_x000D_ _x005F_x000D_
Expression system	Mammalian cells
Accession	Q8TDQ1
Protein length	Thr20-Leu155
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	18.60 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	CMRF35-like molecule 1, CLM-1, CD300 antigen-like family member F, Immune receptor expressed on myeloid cells 1, IREM-1, Immunoglobulin superfamily member 13, IgSF13, NK inhibitory receptor, CD300f, CD300LF, CD300F, CLM1, IGSF13, IREM1, NKIR, UNQ3105/PRO10111
Background	CMRF35-like molecule 1 (CD300f/CD300LF) is a ~32 kDa protein. Acts as an inhibitory receptor for myeloid cells and mast cells. Positively regulates the phagocytosis of apoptotic cells (efferocytosis) via phosphatidylserine (PS) recognition; recognizes and binds PS as a ligand which is expressed on the surface of apoptotic cells. Plays an important role in the maintenance of immune homeostasis, by promoting macrophage-mediated efferocytosis and by inhibiting dendritic cell-mediated efferocytosis. Negatively regulates Fc epsilon receptor-dependent mast cell activation and allergic responses via binding to ceramide and sphingomyelin which act as ligands. May act as a coreceptor for interleukin 4 (IL-4). _x005F_x000D_ _x005F_x000D_ 1. Alvarez-Errico, D. et al. (2004) European journal of immunology 34, 3690-701. PMID: 15549731 _x005F_x000D_ _x005F_x000D_ 2. Izawa, K. et al. (2014) The Journal of allergy and clinical immunology 133, 270-3.e1-7. PMID: 24035150 _x005F_x000D_ _x005F_x000D_ 3. Kim, EJ. et al. (2012) Immunology 135, 226-35. PMID: 22043923 _x005F_x000D_
Note	For research use only.

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SDS-PAGE

SDS-PAGE for Recombinant Human CD300f/CD300LF Protein, C-His

References

Datasheet

Human CD300f/CD300LF Recombinant Protein (C-His).pdf

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Human CD300f/CD300LF Recombinant Protein (C-His) (HV211011)

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