Human EMP2 Recombinant Protein (N-GST & C-His) (HX012012)

Name: Human EMP2 Recombinant Protein (N-GST & C-His)
Brand: abinScience
SKU: HX012012
Price: 211 USD
Availability: InStock

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Catalog No.	HX012012
Description	Recombinant Human EMP2 Protein, N-GST & C-His (HX012012) expressed in E. coli, spanning Ile21-Thr67. Purity: >90% by SDS-PAGE. Highlights ●His-Tagged — N-terminal 6×His tag for IMAC purification. ●E. coli Expression — High-yield, cost-effective production. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	E. coli
Accession	P54851
Protein length	Ile21-Thr67
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Predicted molecular weight	33.54 kDa
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Epithelial membrane protein 2; EMP-2; Protein XMP; EMP2; XMP
Background	Epithelial membrane protein 2 is a ~19 kDa protein. Functions as a key regulator of cell membrane composition by regulating protein surface expression. Also, plays a role in regulation of processes including cell migration, cell proliferation, cell contraction and cell adhesion. Regulates transepithelial migration of neutrophils into the alveolar lumen, potentially via mediation of cell surface expression of adhesion markers and lipid raft formation. Negatively regulates caveolae formation by reducing CAV1 expression and CAV1 amount by increasing lysosomal degradation. Facilitates surface trafficking and formation of lipid rafts bearing GPI-anchor proteins. 1. Gee, HY. et al. (2014) American journal of human genetics 94, 884-90. PMID: 24814193 2. Wadehra, M. et al. (2005) Developmental biology 287, 336-45. PMID: 16216233 3. Morales, SA. et al. (2013) Investigative ophthalmology & visual science 54, 2367-72. PMID: 23439602 4. Fu, M. et al. (2011) PloS one 6, e19945. PMID: 21637765 5. Morales, SA. et al. (2012) Experimental eye research 102, 10-6. PMID: 22728127 6. Morales, SA. et al. (2009) Investigative ophthalmology & visual science 50, 4949-56. PMID: 19494199 7. Wilson, HL. et al. (2002) The Journal of biological chemistry 277, 34017-23. PMID: 12107182 8. Gordon, LK. et al. (2013) Oncogene 32, 5369-76. PMID: 23334331 9. Wadehra, M. et al. (2006) Developmental biology 292, 430-41. PMID: 16487956
Note	For research use only

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SDS-PAGE

SDS-PAGE for Recombinant Human EMP2 Protein, N-GST & C-His

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Datasheet

Human EMP2 Recombinant Protein (N-GST & C-His).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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C₁ × V₁ = C₂ × V₂

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Human EMP2 Recombinant Protein (N-GST & C-His) (HX012012)

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