Anti-Human GITR/TNFRSF18 Reference Antibody (JNJ-64164711, RUO) (HV475086)

Name: Anti-Human GITR/TNFRSF18 Reference Antibody (JNJ-64164711, RUO)
Brand: abinScience
SKU: HV475086
Price: 370 USD
Availability: InStock

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Overview

Catalog No.	HV475086
Description	Anti-Human GITR/TNFRSF18 Reference Antibody (JNJ-64164711, RUO) (HV475086) is a human monoclonal antibody detecting GITR/TNFRSF18. Expressed in Mammalian cells.
Species reactivity	Human
Applications	ELISA, Bioactivity: FCM, Functional assay, Research in vivo
Host species	Human
Clonality	Monoclonal
Isotype	IgG
Clone ID	JNJ-64164711
Biological activity	JNJ-64164711 binds human GITR with high affinity (K d = 180 pM), interacts with human FcγRIIIa variants, targets the CRD1 domain of GITR, and does not inhibit GITR-GITRL binding [1]. JNJ-64164711 mediates greater ADCC-driven depletion of GITR + HuT102, KMS-12-BM, RPMI 8226, JJN-3 cell lines, primary activated GITR + T cells, and in vitro expanded Tregs than other clinical-stage GITR agonist monoclonal antibodies [1]. JNJ-64164711 selectively depletes intratumoral effector Tregs in dissociated primary human patient tumor samples [1].
Expression system	Mammalian cells
Nature	Recombinant
Target	Activation-inducible TNFR family receptor, GITR, TNFRSF18, CD357, Tumor necrosis factor receptor superfamily member 18, AITR, Glucocorticoid-induced TNFR-related protein
Endotoxin level	< 10 EU/mg as determined by LAL method.
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9Y5U5
Form	Liquid
Storage buffer	0.01M PBS pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	JNJ-64164711, JNJ64164711, JNJ64164711
Background	Tumor necrosis factor receptor superfamily member 18 (CD357/GITR) is a ~26 kDa protein. Receptor for TNFSF18. Seems to be involved in interactions between activated T-lymphocytes and endothelial cells and in the regulation of T-cell receptor-mediated cell death. Mediated NF-kappa-B activation via the TRAF2/NIK pathway.
References	1. Holland C V, et al. Development of JNJ-64164711, a low fucose anti-GITR antibody for enhanced depletion of tumor regulatory T cells (Tregs)[J]. Cancer Research, 2018, 78(13_Supplement): 3813-3813. [HV475086]
Note	For research use only. Not suitable for clinical or therapeutic use.
Isotype Control	Human IgG1 Recombinant Isotype Control Antibody (13R4) (HV080507)

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SDS-PAGE for Research Grade Anti-Human CD357/TNFRSF18/GITR (JNJ-64164711)

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Datasheet

Anti-Human GITR/TNFRSF18 Reference Antibody (JNJ-64164711, RUO).pdf

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Anti-Human GITR/TNFRSF18 Reference Antibody (JNJ-64164711, RUO) (HV475086)

Overview

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References

Datasheet

SDS