p53 (TP53) Antibodies & Proteins — Tumor Suppressor Research

p53 needs no introduction. Encoded by the TP53 gene, it is the most frequently mutated gene in human cancer — altered in roughly half of all solid tumors. As a transcription factor, wild-type p53 responds to DNA damage, oncogene activation, and other stress signals by inducing cell cycle arrest, DNA repair, or apoptosis. When p53 is mutated, it typically loses this tumor-suppressive function and, in many cases, acquires oncogenic gain-of-function properties that actively promote tumor progression.

abinScience offers a uniquely deep p53 antibody panel that goes beyond standard detection: wild-type p53 antibodies for IHC, WB, and ELISA, mutant-specific antibodies targeting the R175H hotspot with flow cytometry conjugates, a phospho-p53 (pT18) antibody for DNA damage signaling studies, 2 VHH nanobodies for IP and compact-format detection, and recombinant p53 proteins (human and mouse) for assay development.

Why Mutant-Specific p53 Detection Matters

Standard anti-p53 antibodies detect both wild-type and mutant p53 protein. In many tumors, this is fine — missense mutations in TP53 stabilize the protein, causing it to accumulate to high levels that are easily detected by IHC as strong nuclear staining. But this "pan-p53" detection cannot tell you which mutation is present, or distinguish wild-type p53 stabilized by MDM2 inhibitor treatment from mutant p53 stabilized by loss of degradation.

The R175H mutation is the single most common TP53 hotspot — it accounts for approximately 6% of all p53 mutations across cancer types and is especially frequent in ovarian, colorectal, and breast cancers. R175H disrupts the zinc-binding domain, causing a dramatic conformational change that abolishes DNA binding and exposes neoantigenic surfaces not found on wild-type p53.

abinScience offers a recombinant antibody (clone F2) that specifically recognizes the R175H mutant conformation and does not cross-react with wild-type p53. This antibody is available in 5 conjugate formats (unconjugated + FITC/PE/APC/PerCP) for flow cytometry-based detection of mutant p53 in dissociated tumor cells and cell lines.

Available Products

Wild-Type p53 Detection Antibodies

For general p53 detection (wild-type + mutant) by IHC, WB, and ELISA.

p53 R175H Mutant-Specific Antibodies

Clone F2 — specifically detects the R175H mutant conformation of p53. Does not cross-react with wild-type p53. Available in multiple flow cytometry conjugates for intracellular staining of mutant p53 in tumor cells.

Phospho-p53 & Nanobody Antibodies

p53/TP53 Recombinant Proteins

p53 in IHC: What the Staining Pattern Tells You

p53 IHC is one of the most commonly ordered stains in surgical pathology research, but interpreting it requires understanding what you are actually seeing. The staining pattern reflects the mutation status of TP53 in the tissue:

Note: IHC staining patterns are used as research surrogates for mutation analysis. Definitive mutation identification requires sequencing. All abinScience p53 products are for research use only.

Gene: TP53  |  UniProt: P04637 (Human)  |  Molecular weight: ~43.7 kDa (runs at ~53 kDa on SDS-PAGE due to proline-rich region)  |  Hotspot mutations: R175H, R248W, R273H, G245S, R249S

For research use only. Not intended for diagnostic or therapeutic use.