Anti-Human CTSB Antibody (2A2) (HY436013)

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1mg

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Overview
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Datasheet

Overview

Catalog No.

HY436013

Description

Anti-Human CTSB Antibody (2A2) [2A2] (HY436013) is a mouse monoclonal antibody detecting CTSB in WB. Suitable for Human.

Species reactivity

Human

Applications

Host species

Mouse

Isotype

IgG2a, kappa

Clone ID

2A2

Clonality

Monoclonal

Target

APP secretase, CPSB, Cathepsin B, Cathepsin B1, CTSB, APPS

Endotoxin level

Please contact with the lab for this information.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P07858

RRID

Anti-Human CTSB Antibody (2A2) (abinScience Cat# HY436013, RRID:AB_3728963)

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Cathepsin B (CTSB) is a ~37 kDa protein. Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Cleaves matrix extracellular phosphoglycoprotein MEPE. Involved in the solubilization of cross-linked TG/thyroglobulin in the thyroid follicle lumen. Has also been implicated in tumor invasion and metastasis.

1. Guo, R. et al. (2002) Biochemical and biophysical research communications 297, 38-45. PMID: 12220505

2. Ritonja, A. et al. (1985) FEBS letters 181, 169-72. PMID: 3972105

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-Human CTSB Antibody (2A2).
Flow CytoMetry

Flow-cytometry using FITC anti-human CTSB antibody. HepG-2 cells were fixed and permeabilized, then stained with an irrelevant antibody (Blue Histogram) or an FITC anti-human CTSB monoclonal antibody (Catalog: HY436013, Yellow Histogram) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.
Western blot

Western blot analysis was performed using anti-CTSB monoclonal antibody at 1μg/ml on various samples.
Lane 1: recombinant human CTSB (Catalog No: HY436012), 50ng
Lane 2: recombinant human CTSB (Catalog No: HY436012), 30ng
Lane 3: recombinant human CTSB (Catalog No: HY436012), 20ng