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Anti-AAV2 VP1 Recombinant Antibody (SAA0358) (VK683013)

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Anti-AAV2 VP1 Recombinant Antibody (SAA0358)
Anti-AAV2 VP1 Recombinant Antibody (SAA0358)
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Overview
Catalog No.VK683013
Description
Anti-AAV2 VP1 Recombinant Antibody (SAA0358) (VK683013) is a mouse monoclonal antibody detecting VP1 in ELISA, IF. Suitable for Adeno-associated virus 2 (AAV2).
Highlights
  • ●Affinity Purified — Minimal background and high purity for reliable results.
  • ●Multi-Application — Validated across multiple applications.
Species reactivityAdeno-associated virus 2 (AAV2)
ApplicationsELISA, IF
Host speciesMouse
ClonalityMonoclonal
IsotypeIgG2a, kappa
Clone IDSAA0358
Target Capsid protein VP1, VP1
Endotoxin level < 10 EU/mg as determined by LAL method.
Purity >95% as determined by SDS-PAGE.
Purification Protein A/G purified from cell culture supernatant.
Accession P03135
RRID Anti-AAV2 VP1/Capsid protein VP1 Antibody (SAA0358) (abinScience Cat# VK683013, RRID:AB_3725875)
Form Liquid
Storage buffer 0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Product Usage Information
Application Dilution
ELISA 1:2000-1:20000
Stability and Storage Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.
Background

Capsid protein VP1 is a ~81 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s).

Note For research use only.
Images
  • Anti-AAV2 VP1 Recombinant Antibody (SAA0358)

    SDS-PAGE

    SDS-PAGE for Anti-AAV2 VP1/Capsid protein VP1 Antibody (SAA0358).

References
Datasheet
Download SDS PDF
Formula
Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)
Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.
Mass
=
Concentration
×
Volume
Molecular Weight *
g/mol
Formula
C₁ × V₁ = C₂ × V₂
Enter any 3 of the 4 values to solve for the unknown.
Stock Solution
C₁ (Stock Conc.)
×
V₁ (Stock Vol.)
=
Working Solution
C₂ (Working Conc.)
×
V₂ (Working Vol.)
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Anti-AAV2 VP1 Recombinant Antibody (SAA0358)

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