Anti-AAV2 VP1/Capsid protein VP1 Polyclonal Antibody (VK683014)

Name: Anti-AAV2 VP1/Capsid protein VP1 Polyclonal Antibody
Brand: abinScience
SKU: VK683014
Price: 168 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK683014

Species reactivity

Adeno-associated virus 2

Applications

ELISA, IHC, WB

Host species

Rabbit

Isotype

IgG

Clonality

Polyclonal

Immunogen

E. coli - derived recombinant AAV-2 VP1/Capsid protein VP1 (Gly226-Gln461).

Target

Capsid protein VP1, VP1 (isolate Srivastava/1982) (AAV-2)

Purification

Purified by antigen affinity column.

Accession

P03135

Form

Liquid

Storage buffer

0.01M PBS, pH 7.4, 50% Glycerol, 0.05% Proclin 300.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:5000-1:20000
IHC	1:50-1:500
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.

Background

Capsid protein VP1 is a ~81 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s).

Note

For research use only.

Images

Western blot

Western blot analysis was performed using anti-AAV2 VP1/Capsid protein VP1 polyclonal antibody at 1ug/mL on recombinant AAV2 VP1/Capsid protein VP1 (Catalog No. VK683012).

References

Datasheet