Anti-AAV2 VP1 Recombinant Antibody (SAA0362) (VK683023)

Name: Anti-AAV2 VP1 Recombinant Antibody (SAA0362)
Brand: abinScience
SKU: VK683023
Price: 126 USD
Availability: InStock

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Overview
Images
References
Datasheet
SDS

Overview

Catalog No.

VK683023

Description

Anti-AAV2 VP1 Recombinant Antibody (SAA0362) (VK683023) is a human monoclonal antibody detecting VP1 in ELISA, WB. Suitable for Adeno-associated virus 2 (AAV2).

Highlights

●Affinity Purified — Minimal background and high purity for reliable results.
●Multi-Application — Validated across multiple applications.

Species reactivity

Adeno-associated virus 2 (AAV2)

Applications

ELISA, WB

Host species

Human

Clonality

Monoclonal

Isotype

IgG1, kappa

Clone ID

SAA0362

Target

Capsid protein VP1, VP1

Endotoxin level

< 10 EU/mg as determined by LAL method.

Purity

>95% as determined by SDS-PAGE.

Purification

Protein A/G purified from cell culture supernatant.

Accession

P03135

RRID

Anti-AAV2 VP1/Capsid protein VP1 Antibody (SAA0362) (abinScience Cat# VK683023, RRID:AB_3725904)

Form

Liquid

Storage buffer

0.01M PBS pH 7.4.

Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.

Product Usage Information

Application	Dilution
ELISA	1:2000-1:20000
WB	1:500-1:2000

Stability and Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store at 4°C short term (1-2 weeks). Store at -20°C 12 months. Store at -80°C long term.

Background

Capsid protein VP1 is a ~81 kDa protein. Capsid protein self-assembles to form an icosahedral capsid with a T=1 symmetry, about 22 nm in diameter, and consisting of 60 copies of three size variants of the capsid protein VP1, VP2 and VP3 which differ in their N-terminus. The capsid encapsulates the genomic ssDNA. Binds to host cell heparan sulfate and uses host ITGA5-ITGB1 as coreceptor on the cell surface to provide virion attachment to target cell. This attachment induces virion internalization predominantly through clathrin-dependent endocytosis. Binding to the host receptor also induces capsid rearrangements leading to surface exposure of VP1 N-terminus, specifically its phospholipase A2-like region and putative nuclear localization signal(s).

Note

For research use only.

Images

SDS-PAGE

SDS-PAGE for Anti-AAV2 VP1/Capsid protein VP1 Antibody (SAA0362).

References

Datasheet

Anti-AAV2 VP1 Recombinant Antibody (SAA0362).pdf

Download SDS PDF

Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

Enter any 2 of Mass, Concentration, Volume + Molecular Weight to solve for the unknown.

Mass

Concentration

Volume

Molecular Weight *

g/mol

Formula

C₁ × V₁ = C₂ × V₂

Enter any 3 of the 4 values to solve for the unknown.

Stock Solution

C₁ (Stock Conc.)

V₁ (Stock Vol.)

Working Solution

C₂ (Working Conc.)

V₂ (Working Vol.)

Recombinant Proteins

Antibodies

Assay Kits

Anti-AAV2 VP1 Recombinant Antibody (SAA0362) (VK683023)

Overview

Images

References

Datasheet

SDS