InVivoMAb Anti-Mouse PD-L1/B7-H1 Antibody (10F.9G2) (MV974020)

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100ug
1mg

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SDS

Overview

Catalog No.	MV974020
Description	InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody (10F.9G2) [10F.9G2] (MV974020) is a rat monoclonal antibody detecting Programmed cell death 1 ligand 1 in in vivo PD-L1 blockade, in vitro PD-L1 blockade, IF, IHC-F, FCM, WB. Suitable for Mouse. Highlights ●Multi-Application — Validated across multiple applications. ●Flow Cytometry — Validated for flow cytometry applications.
Species reactivity	Mouse
Applications	in vivo PD-L1 blockade, in vitro PD-L1 blockade, IF, IHC-F, FCM, WB
Host species	Rat
Isotype	IgG2b
Clone ID	10F.9G2
Clonality	Monoclonal
Target	Programmed cell death 1 ligand 1, PD-L1, PDCD1 ligand 1, Programmed death ligand 1, B7 homolog 1, B7-H1, CD274, Cd274, B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Endotoxin level	< 1EU/mg
Purity	>95% as determined by SDS-PAGE.
Purification	Protein A/G purified from cell culture supernatant.
Accession	Q9EP73
Form	Liquid
Storage buffer	0.01M PBS, pH 7.4. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Background	Programmed cell death 1 ligand 1 (CD274/PD-L1) is a ~32 kDa protein. Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response. Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10). CD274 is the therapeutic target of atezolizumab (Tecentriq) and durvalumab (Imfinzi). 1. Tamura, H. et al. (2001) Blood 97, 1809-16. PMID: 11238124 2. Freeman, GJ. et al. (2000) The Journal of experimental medicine 192, 1027-34. PMID: 11015443 3. Wang, S. et al. (2003) The Journal of experimental medicine 197, 1083-91. PMID: 12719480
Note	For research use only. Not suitable for clinical or therapeutic use.

Images

Bioactivity

Detects Recombinant Mouse CD274/PD-L1/B7-H1 Protein (Cat No.: MV974011) in indirect ELISA.
SDS-PAGE

SDS-PAGE for InVivoMAb Anti-Mouse CD274/PD-L1/B7-H1 Antibody (10F.9G2).
Flow CytoMetry

Flow-cytometry using FITC anti-Mouse PD1 antibody. Unstimulated cells (blue Histogram) and 3-day CoA-stimulated Mouse spleen cells (Yellow Histogram) were stained with an FITC anti-Mouse PD1 monoclonal antibody (Catalog: MV974020) at a concentration of 5 µg/ml for 30 mins at RT. After washing, cells analysed on a NovoCyte Flow Cytometer.