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Human NGF/Beta-NGF Recombinant Protein (C-Fc) (HF909011)

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Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

Human NGF/Beta-NGF Recombinant Protein (C-Fc)

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Overview
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References
Datasheet
SDS

Overview

Catalog No.	HF909011
Description	Recombinant Human NGF/Beta-NGF Protein, C-Fc (HF909011) expressed in Mammalian Cells, spanning Ser122-Val238. Purity: >90% by SDS-PAGE. Highlights ●Fc Fusion — Enhanced stability with Protein A/G purification compatibility. ●Mammalian Expression — Proper folding and native glycosylation. ●High Purity — >90% purity verified by SDS-PAGE.
Expression system	Mammalian Cells
Accession	P01138
Protein length	Ser122-Val238
Applications	ELISA, Immunogen, SDS-PAGE, WB, Bioactivity testing in progress
Species	Homo sapiens (Human)
Nature	Recombinant
Endotoxin level	Please contact with the lab for this information.
Purity	>90% as determined by SDS-PAGE.
Form	Lyophilized
Storage buffer	Lyophilized from a solution in PBS pH 7.4, 1 mM EDTA, 4% Trehalose, 1% Mannitol. Please refer to the specific buffer information in the hardcopy of datasheet or the lot-specific COA.
Reconstitution	Reconstitute in sterile water for a stock solution. Adjust the volume of sterile water so that, after reconstitution, the final stock concentration matches the value indicated on the product label or datasheet. RECONSTITUTION PROTOCOL 1. Allow the product to be reconstituted and the reconstitution diluent to reach room temperature. 2. Tap or briefly centrifuge the product vial before opening to dislodge any lyophilized material that may be dispersed on the wall or cap of the vial. 3. Use the diluent and stock concentration recommended in the product datasheet. If preparing a stock concentration higher than recommended, contact Technical Service. 4. After adding the diluent, re-cap the vial and invert gently by hand or place on a slow rocking platform to allow the reconstitution diluent to coat all the surfaces inside the vial. Note: Do not mix by vortexing or by pipetting the material up and down. 5. Allow the vial to sit at room temperature with gentle agitation for at least 15 minutes before aliquotting or using. 6. Store the reconstituted material in single use aliquots in polypropylene or siliconized tubes. Aliquots of 10 μL or larger are recommended.
Shipping	In general, proteins are provided as lyophilized powder/frozen liquid. They are shipped out with dry ice/blue ice unless customers require otherwise.
Stability and Storage	Use a manual defrost freezer and avoid repeated freeze thaw cycles. Store at 2 to 8°C for frequent use. Store at -20 to -80°C for twelve months from the date of receipt.
Alternate Names	Beta-nerve growth factor, NGFB, Beta-NGF, NGF
Background	Beta-nerve growth factor (NGF/Beta-NGF) is a ~26 kDa protein. Nerve growth factor is important for the development and maintenance of the sympathetic and sensory nervous systems. Extracellular ligand for the NTRK1 and NGFR receptors, activates cellular signaling cascades to regulate neuronal proliferation, differentiation and survival (Probable). The immature NGF precursor (proNGF) functions as a ligand for the heterodimeric receptor formed by SORCS2 and NGFR, and activates cellular signaling cascades that lead to inactivation of RAC1 and/or RAC2, reorganization of the actin cytoskeleton and neuronal growth cone collapse. In contrast to mature NGF, the precursor form (proNGF) promotes neuronal apoptosis (in vitro). Inhibits metalloproteinase-dependent proteolysis of platelet glycoprotein VI. 1. Einarsdottir, E. et al. (2004) Human molecular genetics 13, 799-805. PMID: 14976160 2. Carvalho, OP. et al. (2011) Journal of medical genetics 48, 131-5. PMID: 20978020 3. Wijeyewickrema, LC. et al. (2010) The Journal of biological chemistry 285, 11793-9. PMID: 20164177
Note	For research use only.

Images

SDS-PAGE

SDS-PAGE for Recombinant Human NGF/Beta-NGF Protein, C-Fc
Bioactivity

Detected by Tanezumab (Catalog No: HF909016) in indirect ELISA.
Bioactivity

Detected by Bedinvetmab (Catalog No: CF909016) in indirect ELISA.
Bioactivity

Detected by Izenivetmab (Catalog No: CF909036) in indirect ELISA.
Western blot

Detected by Anti-NGF/Beta-NGF monoclonal antibody (Catalog No: HF909010)

References

HF909011-SDS-PAGE-1.jpg

Datasheet

Human NGF/Beta-NGF Recombinant Protein (C-Fc).pdf

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Formula

Mass (g) = Concentration (mol/L) × Volume (L) × MW (g/mol)

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Formula

C₁ × V₁ = C₂ × V₂

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Stock Solution

C₁ (Stock Conc.)

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Working Solution

C₂ (Working Conc.)

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Human NGF/Beta-NGF Recombinant Protein (C-Fc)